PRIMER_PRODUCT_SIZE_RANGE=150-250 PRIMER_PRODUCT_OPT_SIZE=200
SEQUENCE_INTERNAL_OLIGO_MIN_SIZE=18 SEQUENCE_INTERNAL_OLIGO_OPT_TM=55.0 Save this as my_primers.txt : primer3 input
SEQUENCE_ID=TP53_exon7_test SEQUENCE_TEMPLATE=GGACCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGAAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCCTGCACCAGCAGCTCCTACACCGGCGGCCCCTGCACCAGCCCCCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCCGTCTGGGCTTCTTGCATTCTGGGACAGCCAAGTCTGTGACTTGCACGGTCAGTTGCCCTGAGGGGCTGGCTTCCATGAGACTTCAT PRIMER_TASK=pick_pcr_primers SEQUENCE_TARGET=120,1 primer3 input